What test systems are needed for my in-house DMPK studies?
You need options; each in vitro assay is used to answer a specific question within a drug’s metabolism and pharmacokinetics, and while some studies require whole cell systems such as hepatocytes, others may be specific to drug metabolizing enzymes and call instead for subcellular fractions like S9 or microsomes to amplify activity.
Our designated Test Systems Specialists & Production team have been perfecting techniques for more than 25 years to isolate hepatocytes and prepare high-quality cell fractions that maintain high activity so you can collect accurate, consistent data predicting your drug’s effects in vivo. In vitro assays comprise a critical component of preclinical drug safety and in the vitro models you select must be reliable to produce data upon which crucial go/no-go decisions are made.
Regulatory authorities’ requirements and recommendations can sometimes present investigators with multiple alternatives for appropriate test systems and methodology, so we’ve put our heads together to compile a simple guide to the different test systems available.
| Applications | In Vitro Test Systems | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Plateable Cryopreserved Hepatocytes | Pooled Cryopreserved Hepatocytes | Individual Cryopreserved Hepatocytes |
Kupffer Cells | Pooled/S9/Cystosol / Homogenate | Pooled Microsomes | Individual Micosomes/S9/Cytosol | Recombinant Enzymes (rCYPS) | Lysosomes/Tritosomes | |
| Species Differences | + | +++ | ++ | – | ++ | ++ | + | ++ | ++ |
| Metabolic Stability | +++ | +++ | ++ | – | ++ | ++ | + | + | +++ |
| In Vitro Toxicity |
++ | – | – | + | +++ | + | – | – | – |
| Enzyme Induction | +++ | – | – | – | – | – | – | – | – |
| P450 Inhibition | + | ++ | + | – | + | +++ | + | ++ | – |
| Reaction Phenotyping | + | + | + | – | ++ | +++ | + | ++ | – |
| Integrated Metabolism | +++ | +++ | ++ | – | ++ | ++ | + | – | + |
| Uptake Transporter Assays | +++ | +++ | ++ | – | – | – | – | – | – |
| Efflux Transporter Assays |
+++ | – | – | – | – | – | – | – | – |
| Genetic Polymorphisms | + | +++ | + | – | – | – | ++ | +++ | – |
| 3D Hepatic Models | ++ | – | ++ | ++ | – | – | – | – | – |
Make the right choices
Read more about the test systems listed above in our test systems guide, “How to Choose the Right Test Systems for Your DMPK Studies”
Learn more about our Test systems
Explore in vitro test systems we offer, including tissue microarrays, hepatocytes, and microsomes, as well as complementary cell media and regeneration systems
