Single donor plateable Human hepatocytes can be used as a test system in many drug metabolism and pharmacokinetics (DMPK)-related preclinical studies (e.g. definitive enzyme induction assays, metabolic stability, integrated metabolism, uptake/efflux transporter assays, 3D hepatic models) as well as other disciplines, such as virology, cell biology, biochemistry, and genomics. We offer CryostaX® single-freeze format with assured minimum yield (AMY) of 5 million cells, or traditional format cryopreserved heaptocytes with AMY of 4 or 6 million cells. We include many selection criteria options, including demographics and characterization data, affording you plenty of lots to choose from to fit your study.
Single Donor Plateable Human Hepatocytes
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Cryoplateable hepatocytes are the closest alternative to using fresh hepatocytes. They are unique in their ability to attach to collagen substratum and can be cultured long enough to respond to prototypical inducers even after being cryogenically frozen. Our products team qualifies every attaching lot for the ability to maintain a confluent monolayer (>85% confluency) in culture for five days, an important criterion for IND-enabling enzyme induction, toxicology, and some low-turnover compound metabolism studies. Additionally, a datasheet detailing optimal seeding density and a photomicrograph of culture accompany each lot shipment for user convenience.
Cryopreservation allows for long-term testing from the same donor while maintaining the ability to respond to prototypical inducers, maintain drug-metabolizing enzyme activities, and express transporter activity. Since these cryopreserved hepatocytes are not as time-sensitive as fresh hepatocytes, they are most commonly used for long-term metabolic stability, induction, toxicity, and transporter studies. This allows you convenience as the end-user, by not having to schedule your studies around the sporadic availability of fresh hepatocytes.
CryostaX® Single Donor Human Hepatocytes
As an alternative to traditional vial format, we offer a convenient format for human and animal cryopreserved primary hepatocytes called CryostaX®. Our patented production technique was developed to minimize the number of freeze-thaw cycles cells have to endure during the pooling process, reducing cryoinjury to improve the quality of your assays. But the resulting format has added benefits; because CryostaX® are cryopreserved in pellet format, AMY per vial can be easily adjusted for customers who need custom cell yields for their assays that require fewer cells. Additionally, the thawing process is simplified (see our demonstration video below) to enable you to go from storage to assay in fewer steps, and our streamlined ordering process gives you all the information you need– including upfront pricing– to choose the perfect lots for your assays.
Hepatocyte Characterization
| Enzyme | Marker Substrate Reaction |
|---|---|
| CYP1A2 | Phenacetin O-dealkylation |
| CYP2A6 | Coumarin 7-hydroxylation |
| CYP2B6 | Bupropion hydroxylation |
| CYP2C8 | Amodiaquine N-dealkylation |
| CYP2C9 | Diclofenac 4′-hydroxylation |
| CYP2C19 | S-Mephenytoin 4′-hydroxylation |
| CYP2D6 | Dextromethorphan O-demethylation |
| CYP2E1 | Chlorzoxazone 6-hydroxylation |
| CYP3A4/5 | Testosterone 6β-hydroxylation |
| CYP3A4/5 | Midazolam 1′-hydroxylation |
| UGT | 7-Hydroxycoumarin glucuronidation |
| SULT | 7-Hydroxycoumarin sulfonation |
| OCT1* | 1-Methyl-4-phenylpyridinium iodine |
| OATP1B1* | Estrone-3-sulfate |
| OATP1B3* | Cholecystokinin-8 |
| NTCP* | Taurocholic acid |
*Uptake transporter characterization available on specified lots.
*Fold Induction for CYP1A2, 2B6 and 3A4 determined by measuring mRNA levels and enzymatic activities.
Thawing & Plating Cryopreserved Hepatocytes
Download our protocol or watch the demos below to get the best results when thawing and plating your cryopreserved hepatocytes:
What Else You Should Know About Attaching Primary Human Hepatocyte Lots…
Cryoplateable hepatocytes are the closest alternative to using fresh hepatocytes. They are unique in their ability to attach to collagen substratum and can be cultured long enough to respond to prototypical inducers even after being cryogenically frozen. Our products team qualifies every attaching lot for the ability to maintain a confluent monolayer (>85% confluency) in culture for five days, an important criterion for IND-enabling enzyme induction, toxicology, and some low-turnover compound metabolism studies. Additionally, a datasheet detailing optimal seeding density and a photomicrograph of culture accompany each lot shipment for user convenience.
Make sure you get the most out of your hepatocytes
When you purchase from us, we make sure you have all the right tools and information you need to succeed
Build Your Own Pool
We offer custom pooling for no additional cost! You can select attaching or suspension lots, pool size, donor criteria, enzyme activity characterization, and more
