Specialized Media to Thaw and Culture Kupffer Cells

Our proprietary Kupffer cell media, K8700.KIT, has been discontinued. However, you may prepare your own media to assist in culturing cryopreserved Kupffer Cells effectively, maximizing viability and activity, by following this recipe:

DMEM, high glucose, HEPES, without phenol red
FBS – 5.0%
Sodium Pyruvate – 1.0 mM
Insulin – 6.3 ug/mL
Sodium Bicarbonate – 44 mM
Glutamax – 2.0 mM
MEM NEAA – 0.1 mM

Medium needs to be made in sterile conditions using cell culture certified supplements. If customer choose not to use cell culture certified media supplements, the media will need to be filter sterilized (0.25um) after supplementation with the various components.

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Kupffer cells are resident macrophage, non-parenchymal cells found in the liver and can be used in assays by themselves or with hepatocytes in cocultures to improve the representation of cultures of in vivo conditions. To get the best post-thaw performance out of XenoTech Kupffer cells, we recommend using our specialized Kupffer cell medium, a nutrient-rich formula specially tailored to improve thawing and culturing Kupffer cells.

Utilizing our media to get the most out of your Kupffer cells and cocultures

Pro-inflammatory cytokines released by activated Kupffer cells, such as TNF-α and IL-6, are associated with the up-regulation of acute-phase response proteins and suppression of CYP enzymes¹. Our team investigated the potential of Kupffer cell activation in a study where Kupffer cells were isolated from non-transplantable human livers followed by expansion in culture and evaluation of the cytokine response to endotoxin (E. coli lipopolysaccharide, LPS).

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CYP inhibition medium-throughput screening assays offer drug developers data quickly to determine if their drug candidate may down-regulate drug-metabolizing enzymes

Our expert DMPK product specialists are ready to help you get what you need for high performance in vitro assays with dependable results

References

Mallick, Pankajini, et al. “Regulation of Drug-Metabolizing Enzymes in Infectious and Inflammatory Disease: Implications for Biologics–Small Molecule Drug Interactions.” Expert Opinion on Drug Metabolism & Toxicology, vol. 13, no. 6, 2017, pp. 605–616., doi:10.1080/17425255.2017.1292251.

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Make sure you have the right tools and handling procedures in place to get accurate data from your Kupffer cells and cocoultures

Kupffer Cell Thawing and Plating Procedure